16 AUG 2017 · Human monoclonal antibody (mAbs) are emerging in the field of cancer therapy and have become an increasing proportion of new drugs that are recently approved. Although there are some methods to obtain antigen-specific mAbs from human B cells, it is generally impossible to directly immunize human beings with antigens of interest. For example, for infectious agents, those approaches are largely restricted. To solve these obstacles, two main approaches have been developed; either by humanizing antigen-specific antibodies from small experimental animals (which is laborious due to the great genetic differences from humans) or rely on the in vitro selection of antigen-specific binders from human antibody repertoires. However, the human mAbs developed by these methods are usually with low affinity. http://www.creative-biolabs.com/High-Affi-TM-Human-Antibody-Discovery.html

16 AUG 2017 · By introducing human immunoglobulin loci (heavy and light chains) into the mouse germlines with inactivated/disrupted endogenous antibody machinery, the transgenic mouse/rat strains can directly generate high affinity, fully human antibodies, which avoid the subsequent costly and tedious in vitro humanization and affinity maturation process. Upon immunization, the Ig transgenes will undergo V(D)J rearrangement, random nucleotide (N region) addition, class switching, and somatic hypermutation to yield impeccable antibodies. This technology takes advantage of the naturally intact immune system with robust response, high-efficient selection and in vivo maturation while maintaining fully humanness which can eliminate immunogenicity concerns. http://www.creative-biolabs.com/Human-Antibody-Production-from-Transgenic-Mice.html

16 AUG 2017 · Neoangiogenesis is very important for tumor development. Both blood and lymphatic vessels of tumor express unique markers that distinguish them from normal tissue vasculatures. According to the vascular heterogeneity and changed microenvironment, tumor vasculatures show multiple alterations and differences with regular tissue vasculatures, such as abnormal vessel wall structures, leakiness to macromolecules and constant formation and/or remodeling of vessels. Relevant studies have shown that the endothelial cells of tumor are involved in the variable expression of cell surface molecules by expressing a stimulated phenotype. http://www.creative-biolabs.com/In-vivo-Phage-Display-for-Tumor-Targeting.htm

16 AUG 2017 · In vivo phage display is a unique high-throughput method for biopanning by which the peptide phage libraries are directly injected into animals for isolating and identifying the phages bound to specific tissues. Compared with other panning methods, the targets used for isolating phage-displayed peptides are more “intact” with the natural molecular structure and are in the functional states in vivo. Those selected peptides can be used as selective protein transduction domains (PTD) or cell-penetrating peptides (CPPs) for transporting chemotherapeutic agents to the specific tissue, thus improving drug specificity and efficacy in reducing toxicity. Furthermore, some selected peptides can block the function of their ligand proteins by themselves. Using this method, we can offer the functional analysis of new receptors and potential identification and development of novel drug target candidates for the customers. http://www.creative-biolabs.com/In-vivo-Phage-Display-Service.htm

16 AUG 2017 · The cartilaginous fishes are known as the oldest phylogenetic group from evolutionary perspective in which possess a “modern” human-type adaptive immune system and immunoglobulins. As one typical species in this family, sharks have been around for over 500 million years. Due to survive in marine environment, large quantities of urea are contained in sharks’ blood to prevent dehydration, while also make their antibodies much more robust than that of other vertebrates. Comparing with the various immunoglobulin isotypes produced by other species, sharks, on the other hand, mainly produce IgM, IgW and IgNAR (immunoglobulin new antigen receptor). IgNAR is a heavy chain homodimer, which consists of five constant domains and one variable region (VNAR) for each heavy chain. http://www.creative-biolabs.com/Monoclonal-Shark-Antibody-Production.html

10 JUL 2017 · Creative Biolabs together with partners who are leading scientists in monoclonal chicken IgY antibody field offers the most recognized custom monoclonal chicken IgY antibody production services. Chicken IgY antibodies are avian equivalents of mammalian IgG antibodies. Compared to mammalian IgG antibodies, chicken IgY antibodies have higher avidity, less cross-reactivity and higher specificity, rendering chicken IgY antibodies more valuable in laboratory experiments and diagnostic assays. http://www.creative-biolabs.com/igY-antibody-generation.html

10 JUL 2017 · In contrast to rabbit hybridoma technology available in other leading companies, Creative Biolabs has built up a unique rabbit monoclonal antibody production platform based on our unparalleled expertise in phage display antibody library technology. We generate rabbit antibody libraries and select high affinity antigen-specific antibodies by phage display following Dr. Christoph Rader's methodology. http://www.creative-biolabs.com/production-of-monoclonal-rabbit-antibody.html

10 JUL 2017 · In 95% of T cells, this consists of an α and β chain, whereas in 5% of T cells this consists of γ and δ chains. This ratio changes during ontogeny and in diseased states. So the idiotype of each V region of a single immunoglobulin molecule may be comprised of as many as 15–20 idiotopes, which can be distinguished by monoclonal anti-idiotypic antibodies or defined by a specific and unique amino acid sequence. http://www.creative-biolabs.com/anti-idiotypic-antibodies-production-service-detecting-t-cell-receptors.html

10 JUL 2017 · The mouse monoclonal antibodies are widely used for therapeutics and pathology researches, but may sometime be limited to their specificity and affinity. http://www.creative-biolabs.com/Native-TM-Mouse-Antibody-Discovery-Service.html

10 JUL 2017 · By merging the ultralong CDR3s discovered in bovine with human IgG scaffolds, novel therapeutic antibodies against challenging hot antigen families, including GPCRs and other intra-membrane targets on the cell surface are possible. Humanized IgG scaffolds that accommodate ultralong CDR3s may target small proteins with desirable therapeutic properties, which are challenging for conventional antibodies. http://www.creative-biolabs.com/Humanization-of-Bovine-Ultralong-CDR3-Antibodies.html