Protein Purification
Aug 16, 2019 ·
53s
Reagents and apparatus of Protein Expression, Isolation and Purification Reagents LB fluid medium: Tryptone 10g, yeast extract 5g, NaCl 10g.Mix all together with distilled water to 1000mL. Ampicillin: 100mg /...
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Reagents and apparatus of Protein Expression, Isolation and Purification
Reagents
LB fluid medium: Tryptone 10g, yeast extract 5g, NaCl 10g.Mix all together with distilled water to 1000mL.
Ampicillin: 100mg / mL
Sample: Buffer Solution. 100 mM NaH2PO4, 10 mMTris, 8M Urea, 10 mM2-ME, pH8.0
Washing Buffer: 100 mM NaH2PO4, 10 mM Tris, 8 M Urea, pH6.3
Elution Buffer: 100mM NaH2PO4, 10 mMTris, 8M Urea, 500 mM Imidazole, pH8.0
IPTG https://www.creative-biolabs.com/blog/index.php/protein-expression-isolation-and-purification/
show less
Reagents
LB fluid medium: Tryptone 10g, yeast extract 5g, NaCl 10g.Mix all together with distilled water to 1000mL.
Ampicillin: 100mg / mL
Sample: Buffer Solution. 100 mM NaH2PO4, 10 mMTris, 8M Urea, 10 mM2-ME, pH8.0
Washing Buffer: 100 mM NaH2PO4, 10 mM Tris, 8 M Urea, pH6.3
Elution Buffer: 100mM NaH2PO4, 10 mMTris, 8M Urea, 500 mM Imidazole, pH8.0
IPTG https://www.creative-biolabs.com/blog/index.php/protein-expression-isolation-and-purification/
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