The series was produced by Cayuga Productions, Inc., a production company owned and named by Serling. It reflects his background in Central New York State and is named after Cayuga Lake, on which he owned a home, and where Cornell University and Ithaca College are located.Aside from Serling, who wrote or adapted nearly two-thirds of the series' total episodes, writers for The Twilight Zone included leading authors such as Charles Beaumont, Ray Bradbury,[6] Earl Hamner, Jr., George Clayton Johnson, Richard Matheson, Reginald Rose, and Jerry Sohl. Many episodes also featured new adaptations of classic stories by such writers as Ambrose Bierce, Jerome Bixby, Damon Knight, John Collier, and Lewis Padgett.

Twilight Zone's writers frequently used science fiction as a vehicle for social comment, as networks and sponsors who censored controversial material from live dramas were less concerned with seemingly innocuous fantasy and sci-fi stories. Frequent themes on The Twilight Zone included nuclear war, McCarthyism, and mass hysteria, subjects that were avoided on less serious primetime television. Episodes such as "The Monsters Are Due on Maple Street" and "I Am the Night—Color Me Black" offered specific commentary on current events and social issues. Other stories, such as "The Masks", "I Dream of Genie", or "Mr. Denton on Doomsday" were allegories, parables, or fables that reflected the moral and philosophical choices of the characters.