16 AUG 2017 · Although single domain antibodies isolated from antibody libraries derived from gene synthesis and immunized or naïve animals generally exhibit reasonable affinity, it is often desirable to further increase their affinity. Quantitative library sorting by phage display or yeast surface display is used for improving the affinity of single domain antibodies. In particular, we have developed a proprietary DNA mutagenesis technique that is able to create a huge number (e.g. 1010) variants of the parental antibody with defined positions mutated. In combination with our first class phage display antibody library construction and screening technologies, 10-100 fold increase in affinity for parental single domain antibodies of low nM affinity is frequently achieved. This is often possible for other companies for antibodies of 100nM affinity or worse. http://www.creative-biolabs.com/Nanobody-Affinity-Maturation.html

10 JUL 2017 · Creative Biolabs has extensive experience to offer the antibody humanization service for therapeutic and diagnostic development. It has successfully performed 15 mouse/rat humanization projects during the past decade with at least one humanized antibody entered the clinical trials. http://www.creative-biolabs.com/antibody-humanization.html

20 MAR 2017 · To support the manufacture of antibody in various application areas, Creative Biolabs is able to offer comprehensive services for antibody production. From sequence optimization, gene synthesis to antibody expression and purification, we are well-recognized antibody provider for either research quantities or industrial manufacture. Our antibody production services also include both production and purification of bispecific antibody, recombinant IgG, scFv/Fab and single domain antibodies(sdAbs). http://www.creative-biolabs.com/Antibody-Production-Services.html

20 MAR 2017 · Bispecific antibodies (BsAbs) are artificial antibodies which possess two distinct specificities. Thereby, it is able to recognize two different antigens or epitopes simultaneously. Taking advantage of this unique character, researchers use BsAbs to guide effectors to targets of interest for further cellular or physiological effects, such as triggering the contacts between immune cells and tumor cells by binding marker proteins on the surface of each cell type simultaneously. http://www.creativebiolabs.net/bispecific-antibody-fagments-bsab-fragments.htm

15 MAR 2017 · Bispecific antibodies are now being served as effective therapeutic agents in cancer immunotherapy. Certain recombinant strategies have been developed to synthesize bispecific antibodies, which include single chain variable fragment (scFv)-derived formats such as tandem diabodies, diabodies, DARTs (Dual Affinity Re-Targeting), BiTes (bispecific T-cell engager), and , as well as immunoglobulin G(IgG)-based formats such as DVD-Ig (Dual Variable Domain antibodies), Triomab, and two-in-one antibodies. http://www.creativebiolabs.net/Bispecific-Antibodies_12.htm

15 MAR 2017 · Manufacturability assessment consists of both productivity and stability assessment. Productivity is evaluated by which the product is manufactured with the correct structure and post-translational modifications at desired levels from a production cell line, purified in an intact form. During the production, some antibody drug candidates do not express or fold properly and some even cause extensive aggregation in the cell culture media or during the following purification procedures due to the structural modifications. http://www.creative-biolabs.com/Manufacturability-Assessment.htm

15 MAR 2017 · Compared with mouse antibodies, rat monoclonal antibodies offer several advantages, including the reduced antigen requirement, the availability of using mouse antigens and greater lymphocyte yields. Moreover, rat antibodies pair better than that of mouse in sandwich assays: there is no cross reaction of available anti-mouse secondary antibody binding to the capture rat antibody. http://www.creative-biolabs.com/Native-TM-Rat-Antibody-Discovery-Service.html

15 MAR 2017 · HuScL-1(R), HuScL-2(R), HuScL-3(R) and HuScL-4(R) Human Single Chain (scFv) Antibody Libraries are natural or synthetic libraries of a diversity of over 2.0×109. Recently, we have created a next-generation, fully-human, yeast display scFv library - HuScL-YDP. We also have a HuFabL(R) Human Fab Antibody Library of a size of 2.0×109. Besides human antibody libraries, we have a MuScL(R) Murine Single Chain Antibody Library and a MuFabL(R) Murine Fab Antibody Library. http://www.creative-biolabs.com/premade-human-and-mouse-antibody-libraries.html

27 FEB 2017 · In the antibody manufacturing process, oxidation is possibly caused by ROS and exposure to light. Therefore, the process conditions must be carefully selected to avoid the risks. The solvent exposed Methionine and Tryptophan should be taken into consideration during the antibody candidate selection to minimize the oxidation potential. http://www.creative-biolabs.com/Oxidation.htm

27 FEB 2017 · Magic™ DNA transfection reagent, which mainly based on a novel synthetic derivative from natural compounds, is particularly suitable for DNA delivery, and has shown outstanding transfection efficiency for both adherent cells and suspension cells. http://www.creative-biolabs.com/magic-dna-transfection-reagent.html