6 AUG 2022 · Hi everyone and welcome back to my final episode for this series this is a bonus episode for the scientists out there who are interested in my masters research project investigating the genetic determinants of IgE mediated food allergy. I sharea a recording of my poster presentation that I had to complete online due to the Covid lockdown in 2020. It gives you a glimpse of the research that I conducted in my Msci year and after I explain the recording. Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word bonus episode 7 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top

20 APR 2022 · Hi everyone and welcome back to episode 6 in this episode I’m joined by my friend Sonia as we discuss her growing up with a brother who has a nut allergy as well her experience having lactose intolerance. We will be sharing some stories with you and just having a chat (very informal). Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word episode 6 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top References/ useful websites: https://www.nhs.uk/conditions/food-intolerance/ https://www.nhs.uk/conditions/lactose-intolerance/ https://www.nhs.uk/conditions/food-allergy/ https://www.allergyuk.org/about-allergy/allergy-vs-intolerance/ https://www.anaphylaxis.org.uk/knowledgebase/food-intolerance/#:~:text=The%20Anaphylaxis%20Campaign%20is%20the,latex%2C%20drugs%20and%20insect%20stings. https://www.bsaci.org/patients/most-common-allergies/food-allergy-and-food-intolerance/#:~:text=The%20commonest%20foods%20causing%20FA,food%20however%2C%20small%20the%20amount. https://www.worldallergy.org/education-and-programs/education/allergic-disease-resource-center/professionals/food-allergy https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6682924/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7551416/ https://www.newscientist.com/article/dn1787-genetic-basis-for-lactose-intolerance-revealed/#:~:text=A%20quick%20and%20cheap%20genetic,identify%20people%20with%20lactose%20intolerance.&text=The%20discovery%20supports%20the%20theory,an%20adaptation%20to%20dairy%20farming. https://www.sciencedaily.com/releases/2020/09/200903114212.htm https://www.npr.org/sections/thesalt/2012/12/27/168144785/an-evolutionary-whodunit-how-did-humans-develop-lactose-tolerance?t=1644334343550 https://www.nature.com/scitable/topicpage/evolutionary-adaptation-in-the-human-lineage-12397/

23 NOV 2021 · Hi everyone and welcome back to episode 5 in this episode I’m joined by my childhood friend Gadah as we discuss growing up in a middle eastern culture whilst having food allergies, tips for travelling for food allergies and how friend can be our biggest supporters when it comes to dealing with food allergies. We will be sharing some stories with you and just having a chat (very informal). Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word episode 5 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top References: https://www.thesun.co.uk/travel/10938777/nut-allergy-sufferer-ryanair/ https://www.manchestereveningnews.co.uk/news/greater-manchester-news/nut-allergy-sufferer-claims-ryanair-17727181 https://thetab.com/uk/manchester/2020/02/14/ryanair-refused-to-stop-selling-kinder-buenos-despite-customer-with-deadly-nut-allergy-45766

23 APR 2021 · Hi everyone and welcome back to episode 4. In this episode I discuss findings from a recent publication that I was involved creating communication material for. The study set out to identify trends in food-induced anaphylaxis hospital admissions and related fatalities from 1998 to 2018 in the UK and I discuss some of the main findings. Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word episode 4 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top References: 1.Schäfer, T., E. Böhler, S. Ruhdorfer, L. Weigl, D. Wessner, J. Heinrich, B. Filipiak, H‐E. Wichmann, and J. Ring. "Epidemiology of food allergy/food intolerance in adults: associations with other manifestations of atopy." Allergy 56, no. 12 (2001): 1172-1179. 2.Brown, A.F., McKinnon, D. and Chu, K., 2001. Emergency department anaphylaxis: a review of 142 patients in a single year. Journal of Allergy and Clinical Immunology, 108(5), pp.861-866. 3.Løvik, M., Namork, E., Fæste, C. and Egaas, E., 2004. The Norwegian national reporting system and register of severe allergic reactions to food. Norsk epidemiologi, 14(2). 4.Afify, S.M. and Pali-Schöll, I., 2017. Adverse reactions to food: the female dominance–A secondary publication and update. World Allergy Organization Journal, 10(1), pp.1-8. 5.Conrado, A.B., Ierodiakonou, D., Gowland, M.H., Boyle, R.J. and Turner, P.J., 2021. Food anaphylaxis in the United Kingdom: analysis of national data, 1998-2018. bmj, 372. 6.Articles links: https://food.blog.gov.uk/2021/02/18/food-anaphylaxis-in-the-uk-what-weve-learnt-by-analysing-national-data/ 7.Article link: https://www.newfoodmagazine.com/article/141009/fsa-takeaway-allergen-research/

21 MAR 2021 · Welcome back to episode 3. Numerous risk factors have been proposed to contribute to food allergy or sensitization, in this episode, I will address a few of the most researched genetic and environmental risk factors that increase the risk of developing IgE-mediated food allergy. If you would like to find out more please do visit my blog post which will detail everything that was discussed in the podcast. You can find the blog post based on this podcast on my website under blogs. Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word episode 2 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top Reference list Allen, K.J., Koplin, J.J., Ponsonby, A.L., Gurrin, L.C., Wake, M., Vuillermin, P., Martin, P., Matheson, M., Lowe, A., Robinson, M. and Tey, D., (2013). Vitamin D insufficiency is associated with challenge-proven food allergy in infants. Journal of allergy and clinical immunology, 131(4), pp.1109-1116. Asai, Y., Eslami, A., van Ginkel, C.D., Akhabir, L., Wan, M., Ellis, G., Ben-Shoshan, M., Martino, D., Ferreira, M.A., Allen, K. and Mazer, B., (2018a). Genome-wide association study and meta-analysis in multiple populations identifies new loci for peanut allergy and establishes C11orf30/EMSY as a genetic risk factor for food allergy. Journal of Allergy and Clinical Immunology, 141(3), pp.991-1001. Asai, Y., Eslami, A., Van Ginkel, C.D., Akhabir, L., Wan, M., Yin, D., Ellis, G., Ben-Shoshan, M., Marenholz, I., Martino, D. and Ferreira, M.A., (2018b). A Canadian genome-wide association study and meta-analysis confirm HLA as a risk factor for peanut allergy independent of asthma. Journal of Allergy and Clinical Immunology, 141(4), pp.1513-1516. Chalmers JR, Haines RH, Mitchell EJ, Thomas KS, Brown SJ, Ridd M, et al. Effectiveness and cost-effectiveness of daily all-over-body application of emollient during the first year of life for preventing atopic eczema in high-risk children (The BEEP trial): protocol for a randomised controlled trial. Trials 2017;18:343. Crespo JF, James JM, Fernandez-Rodriguez C, Rodriguez J. Food Allergy: Nuts and Tree Nuts. Br J Nutr. 2006;96 2:S95–102. Feeney, M., Du Toit, G., Roberts, G., Sayre, P.H., Lawson, K., Bahnson, H.T., Sever, M.L., Radulovic, S., Plaut, M., Lack, G. and Chan, S., 2016. Impact of peanut consumption in the LEAP study: feasibility, growth, and nutrition. Journal of Allergy and Clinical Immunology, 138(4), pp.1108-1118. Grimshaw, K. E., Bryant, T., Oliver, E. M., Martin, J., Maskell, J., Kemp, T., Clare Mills, E. N., Foote, K. D., Margetts, B. M., Beyer, K., & Roberts, G. (2016). Incidence and risk factors for food hypersensitivity in UK infants: results from a birth cohort study. Clinical and translational allergy, 6, 1. https://doi.org/10.1186/s13601-016-0089-8 Lack, G., 2008. Epidemiologic risks for food allergy. Journal of Allergy and Clinical Immunology, 121(6), pp.1331-1336. Marenholz, I., Grosche, S., Kalb, B., Rüschendorf, F., Blümchen, K., Schlags, R., Harandi, N., Price, M., Hansen, G. & Seidenberg, J. (2017). 'Genome-wide association study identifies the SERPINB gene cluster as a susceptibility locus for food allergy', Nature communications, 8(1), pp. 1-10. Matsui, T., Tanaka, K., Yamashita, H., Saneyasu, K.-i., Tanaka, H., Takasato, Y., Sugiura, S., Inagaki, N. & Ito, K. (2019). 'Food allergy is linked to season of birth, sun exposure, and vitamin D deficiency', Allergology International, 68(2), pp. 172-177. Perkin MR, Logan K, Marrs T, Radulovic S, Craven J, Flohr C, et al. Enquiring About Tolerance (EAT) study: feasibility of an early allergenic food introduction regimen. J Allergy Clin Immunol 2016a 137:1477-86. Perkin MR, Logan K, Tseng A, Raji B, Ayis S, Peacock J, et al. Randomized trial of introduction of allergenic foods in breast-fed infants. N Engl J Med 2016b ;374: 1733-43. Perkin, M.R., Logan, K., Marrs, T., Radulovic, S., Craven, J., Boyle, R.J., Chalmers, J.R., Williams, H.C., Versteeg, S.A., Van Ree, R. and Lack, G., 2021. Association of frequent moisturizer use in early infancy with the development of food allergy. Journal of Allergy and Clinical Immunology, 147(3), pp.967-976. Savilahti, E. M., Ilonen, J., Kiviniemi, M., Saarinen, K. M., Vaarala, O. & Savilahti, E. (2010). 'Human leukocyte antigen (DR1)-DQB1* 0501 and (DR15)-DQB1* 0602 haplotypes are associated with humoral responses to early food allergens in children',International archives of allergy and immunology,152(2), pp. 169-177.si Schoemaker, A. A., Sprikkelman, A. B., Grimshaw, K. E., Roberts, G., Grabenhenrich, L., Rosenfeld, L., Siegert, S., Dubakiene, R., Rudzeviciene, O. & Reche, M. (2015). 'Incidence and natural history of challenge‐proven cow's milk allergy in European children–EuroPrevall birth cohort',Allergy,70(8), pp. 963-972. Sicherer, S. H., Furlong, T. J., Maes, H. H., Desnick, R. J., Sampson, H. A. & Gelb, B. D. (2000). 'Genetics of peanut allergy: a twin study',Journal of Allergy and Clinical Immunology,106(1), pp. 53-56. Tordesillas, L.,Berin,M. C. & Sampson, H. A. (2017). 'Immunology of foodallergy', Immunity,47(1), pp. 32-50. Van Ginkel, C.D., Flokstra‐de Blok, B.M.J., Kollen, B.J., Kukler, J., Koppelman, G.H. and Dubois, A.E.J., (2015). Loss‐of‐function variants of the filaggrin gene are associated with clinical reactivity to foods. Allergy, 70(4), pp.461-464. Venkataraman, Devasmitha et al. “Filaggrin loss-of-function mutations are associated with food allergy in childhood and adolescence.” The Journal of allergy and clinical immunology vol. 134,4 (2014): 876-882.e4. doi:10.1016/j.jaci.2014.07.033 Visscher, P.M., Wray, N.R., Zhang, Q., Sklar, P., McCarthy, M.I., Brown, M.A. and Yang, J., (2017). 10 years of GWAS discovery: biology, function, and translation.The American Journal of Human Genetics,101(1), pp.5-22.

21 FEB 2021 · Welcome back to episode 2. In this episode I explain the difference between IgE-mediated food allergy, non IgE mediated food allergy and mixed IgE and non IgE food allergy and I also talk about the immune mechanism that underlies an allergic reaction and what the methods are to diagnosing food allergy with a specific focus on IgE-mediated food allergy in this episode. I also share stories along the way of my personal experiences. If you would like to find out more please do visit my blog post which will detail everything that was discussed in the podcast. You can find the blog post based on this podcast on my website under blogs. Reference list: Aziz, M., Iheanacho, F. and Hashmi, M.F., 2019. Physiology, Antibody. Nicholson, L.B., 2016. The immune system. Essays in biochemistry, 60(3), pp.275-301 Aas, K., 1984. Antigens in food. Nutrition reviews, 42(3), pp.85-91. (https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1753-4887.1984.tb02295.x) Mills, C., 2011. What makes an antigen a food allergen?. Clinical and Translational Allergy, 1(1), pp.1-1. Boyce JA, Assa’ad A, Burks AW, Jones SM, Sampson HA, Wood RA, et al. Guidelines for the diagnosis and management of food allergy in the United States: report of the NIAID-sponsored expert panel. J. Allergy Clin Immunol 2010;126(6 Suppl):S1–58. Burks AW, Tang M, Sicherer S, et al. ICON: Food allergy. J Allergy Clin Immunol 2012, 129:906-20 Connors, L., O’Keefe, A., Rosenfield, L. and Kim, H., 2018. Non-IgE-mediated food hypersensitivity. Allergy, Asthma & Clinical Immunology, 14(2), pp.1-9. Demoly P, Tanno LK, Akdis CA, Lau S, et al. Global classification and coding of hypersensitivity diseases – An EAACI – WAO survey, strategic paper and review. Allergy 2014, 69:559–70 Heinzerling, L., Mari, A., Bergmann, K.C., Bresciani, M., Burbach, G., Darsow, U., Durham, S., Fokkens, W., Gjomarkaj, M., Haahtela, T. and Bom, A.T., (2013). The skin prick test–European standards. Clinical and translational allergy, 3(1), p.3. Roerdink EM, Flokstra-de Blok BM, Blok JL, ET AL. Association of food allergy and atopic dermatitis exacerbations. Ann Allergy Asthma Immunol. 2016;116:334-8 Furuta GT, Katzka DA. Eosinophilic Esophagitis. N Engl J Med 2015;373:1640-8. Yu, W., Freeland, D. M. H. & Nadeau, K. C. (2016). 'Food allergy: immune mechanisms, diagnosis and immunotherapy', Nature Reviews Immunology, 16(12), p. 7 Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Twitter: @WafiAyah Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word episode 2 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top.

1 FEB 2021 · My story is a story of identity. Having food allergies awakened my scientific curiosity, paved education journey and propelled me into a fascinating career. More importantly having food allergies has become an integral part of my identity, it has woven its self into the very fabric that makes me Ayah Wafi. To read more please visit my blog post based on this episode at: https://allergieswithayah.wixsite.com/website/post/food-allergy-is-part-of-my-identity Disclaimer: The information in this podcast is for information and entertainment purposes only. I am not a medical professional so I have never and will never give medical advice. You should always speak to a health care provider about your unique health needs. My opinions are entirely my own. I only discuss published literature in this podcast. I am not responsible for any claims related to the procedures, professionals, products or methods discussed in the podcast, and do does not approve or endorse any products, professionals, services or methods that may be referenced to in this podcast. Instagram: @AllergieswithAyah Website: https://allergieswithayah.wixsite.com/website Image description: The image is an infographic, with a light green background. There is a cartoon mustard tub, celery stick and different types of nuts at the bottom left corner. At the bottom right corner there is a cartoon image of a glass of wine, milk carton, tofu, shellfish, crustacean, purple fish and one egg. On the top left corner there are three wheat stalks and sesame and lupin seeds as cartoon images. On the top right there is a logo with a green antibody in a white box. The logo has the words Allergies at the top of the antibody, the word with to the centre left of the antibody and the word Ayah to the centre right of the antibody. At the centre top of the infographic there is the words Allergies with Ayah in written in grey. The word episode 1 is written to the centre left of Ayah’s face. In the centre of the image there is a cartoon character depiction of Ayah. Ayah has brown long hair and is smiling. Ayah’s hand is together in two fists and she is wearing a purple top. The word episode 1 is written to the centre left of Ayah’s face.