John Owen Lowe is the son of make-up artist Sheryl Berkof and actor Rob Lowe, he graduated in science technology from Stanford University in 2018. He has an older brother named Mathew who works as a lawyer.[ From 2015, Lowe appeared in a recurring role in comedy series The Grinder. In 2017, he appeared on The Lowe Files, in which his family explored unsolved mysteries. He appeared in the Christmas film Holiday in the Wild in 2019, and worked as a writer on multiple episodes of 9-1-1: Lone Star. In March 2023, Lowe appeared in the Netflix comedy series Unstable, which he also executive-produced alongside his father, and Victor Fresco. The scripted series was reportedly inspired by his relationship on social media with his father.