Welcome to the 4th episode of Bytes of Bio, Regulatory Sequence!

Can Italian food help you study biology? Probably. But not here it won't. First out of many terrible metaphors to come.

Also, it might sound like we recorded in a dishwasher at the beginning. But it gets better, I swear. (/ ; ^ ; )/

Let’s go through the main points we have discussed!

- Goal: Locate regulatory sequences, such as the promoters or enhancers, within the gene using histone methylation.

- Definitions:
Regulatory sequences: genetic sequences that regulate the expression of genes.
Histone methylation: a method to regulate genes, using methyl-groups that bind to histone proteins.

- Mapping epigenetic features:
Identify sites of specific histone methylation to locate regulatory sequence.
Extract the aforementioned histones from the genome, and the located regulatory sequence along with it.
Create a map of regulatory sequence locations.
- Predicting promoters based on TSSs and enhancers based on epigenetic features: utilize the results of the map to locate the sequences.

Reference links mentioned throughout the podcast:
Mapping epigenetic feature process (https://imgur.com/a/tUS07iY)
Research on the relation between histone methylation and regulatory sequence (https://academic.oup.com/bioinformatics/article/22/4/392/183869)
ChIP-sequencing (https://en.wikipedia.org/wiki/ChIP-sequencing)

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