Similar to Biacore, ProteOn system detects protein-protein interaction based on SPR technology, which is an extremely sensitive method for detecting molecular interactions by tracking the change of signal measured by sensor chips. Different from using ELISA which is an end-point assay, with ProteOn, you can obtain full kinetic parameters related to the following questions:

How specific is the interaction
How fast is the interaction (Ka)
How stable is the ligand-analyte complex (Kd)
How strong is the affinity (KD)

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