16 AUG 2017 · Guinea pig (Cavia porcellus), which is suggested as domesticated descendants of a closely related species of cavy, is a kind of rodent that do not exist naturally in the wild. It is one of the earliest species that have been applied in the field of research and investigation for more than 200 years. While, since the genetic research of guinea pig has fallen behind during the early 20th century, it becomes less popular than mice and rats in a variety of researches. But guinea pig is still an ideal source to generate antibodies for extreme allergic reactions or anaphylaxis researches, and also be treated as an important model for the study of various infectious diseases, such as tuberculosis and diphtheria. Source: http://www.creative-biolabs.com/Guinea-Pig-Hybridoma-Production.htm

10 JUL 2017 · Single domain antibody, also known as domain antibody, VHH, VNAR or sdAb, is a kind of antibody fragments consisting of a single monomeric variable antibody domain and lacking the light chain and CH domain of the heavy chain in conventional Fab region. The first single domain antibody was engineered from the VHH domain of heavy-chain antibody identified in camelids (e.g. dromedaries, camels, llamas, and alpacas). And the VNAR domain of cartilaginous fishes (e.g. shark) heavy-chain antibody (known as IgNAR, immunoglobulin new antigen receptor) is another qualified source for sdAb development. An alternative approach is to split the dimeric variable domains from normal IgG of humans or mice into monomers by camelizing a few critical residues. Learn more about camelized antibody at http://www.creative-biolabs.com/Nanobody-Single-Domain-Antibody.html.

8 JUN 2017 · Sumoylation is a post-translational modification with the process that SUMO (small ubiquitin-like modifier) proteins are modified on substrate proteins catalyzed by an enzymatic cascade analogous to that involved in ubiquitination. In contrast to ubiquitin, SUMO is not used to tag proteins for degradation but can regulate various protein function including protein-protein interactions and subcellular localization. Sumoylation is also involved in a variety of cellular processes, such as transcriptional regulation, nuclear-cytosolic transport, apoptosis, protein stability, response to stress, and progression through the cell cycle. http://www.creative-biolabs.com/Sumoylation-Specific-Antibody-Production-Service.html

8 JUN 2017 · Affinity maturation is the process to improve antibody affinity for an antigen. In vivo, natural affinity maturation by the immune system takes place by somatic hypermutation and clonal selection. In vitro, in the laboratory affinity maturation, can be obtained by mutation and selection. http://www.creative-biolabs.com/antibody-affinity-maturation.html

8 JUN 2017 · Creative Biolabs offers a high-throughput Magic™ platform for bioactive peptides discovery. This powerful platform permits the rapid characterization of all clones present in the screened library. The goal is to identify one or more families of peptides that specifically bind to the target protein. Then the libraries, which contain a large number of variants of the lead peptide sequences, can be constructed in affinity selective display systems. A number of oligonucleotide mutagenesis strategies are employed to improve the affinity of these peptides. http://www.creative-biolabs.com/Magic-Bioactive-Peptides-Discovery-Service.htm

6 JUN 2017 · Hamsters belong to the largest and most diversified order of Mammalia class, the Rodentia. Like its rodent brothers, rat and mouse, hamster is currently one of the most frequently used lab animals. Monoclonal IgG and IgM of hamster were first produced in the 1980s by creating stable hamster-mouse hybridomas. Although mouse and rat antibodies provide a convenient tool for many applications, the evolutionary proximity of these two species may restrict the antibody responses as they share many conserved antigenic structures. On the other hand, hamster, which is more evolutionarily distant, can be a suitable choice in areas where mouse and rat antibodies might not work very well. http://www.creative-biolabs.com/native-tm-hamster-antibody-discovery.html

6 JUN 2017 · Sharks, and other members in Chondrichthyes, are currently recognized as the most evolutionarily ancient animals that rely on antibodies as part of their adaptive immune system. Interestingly, apart from the expected IgM and IgW, cartilaginous fishes also produce a type of ancestral antibody referred to immunoglobulin new antigen receptor (IgNAR). IgNAR is quite unconventional antibody composed of only a homodimeric heavy chain which contains one variable and five constant domains for each chain (Fig. 1). Owe to the distinct structure between shark and human antibody domains, the extremely high stability of IgNAR has been presented. In addition, VNAR is around 10 times smaller than standard antibodies and able to continue to function and exist within very extreme conditions. These properties allow IgNAR to play a potential role in pharmaceutical application. http://www.creative-biolabs.com/Native-TM-Shark-Antibody-Discovery-Service.html

6 JUN 2017 · Similar to Biacore, ProteOn system detects protein-protein interaction based on SPR technology, which is an extremely sensitive method for detecting molecular interactions by tracking the change of signal measured by sensor chips. Different from using ELISA which is an end-point assay, with ProteOn, you can obtain full kinetic parameters related to the following questions: How specific is the interaction How fast is the interaction (Ka) How stable is the ligand-analyte complex (Kd) How strong is the affinity (KD) http://www.creative-biolabs.com/antibody-humanization.html

6 JUN 2017 · Creative Biolabs provides unparalleled custom anti-idiotypic antibody (anti-ID Abs) production and downstream assay services to support your study, preclinical and clinical trials and the development of monoclonal antibody drugs. Creative Biolabs is professional in developing high specificity and affinity monoclonal antibodies from various species (including rabbit, chicken, llama, camel, alpaca, cow, dog, mouse, rat, sheep and human). In addition to mouse and rat hybridoma production methods, we have phage display immune antibody library construction and screening methods which are the best method to raise monoclonal antibodies from species that do not have hybridoma technology available. We provide both custom anti-idiotypic antibody production services and products to meet our customers’ needs. http://www.creative-biolabs.com/Production-of-Anti-idiotype-Antibody.html

6 JUN 2017 · Single domain antibody, also known as domain antibody, VHH, VNAR or sdAb, is a kind of antibody fragments consisting of a single monomeric variable antibody domain and lacking the light chain and CH domain of the heavy chain in conventional Fab region. The first single domain antibody was engineered from the VHH domain of heavy-chain antibody identified in camelids (e.g. dromedaries, camels, llamas, and alpacas). And the VNAR domain of cartilaginous fishes (e.g. shark) heavy-chain antibody (known as IgNAR, immunoglobulin new antigen receptor) is another qualified source for sdAb development. An alternative approach is to split the dimeric variable domains from normal IgG of humans or mice into monomers by camelizing a few critical residues. http://www.creative-biolabs.com/Nanobody-Single-Domain-Antibody.html